Refer to contents of the “PCR_Documentation” folder to understand how the API works and what are the requirements to deploy this system. Compile all the essential data of the PCR run into a single, universal file format (json) following a user-friendly scheme to store in on the Covmatic digital server.Interpret the data generated by the PCR from these.csv files are generated which contain the data required to plot these reaction curves and details of the PCR run.
CFX MANAGER SOFTWARE
pcrd (readable by BioRad software – CFX Manager or CFX Maestro) is generated that displays these reaction curves with an interactive interface. The PCR captures the status of this reaction at regular time intervals and generates reaction curves for each well.Īfter the PCR run, a result file of the extension.
Inside the PCR, the samples undergo a thermo-chemical reaction. Now, of course it has 96 samples all from different patients. These wells are labeled A1,A2, A3, B1, B2, B3 …, etc. Imagine that you insert a 96-well plate into a PCR. If you run it from the Covmatic Webserver, then the JSON file generated at the end of the PCR run will be picked up by the server and stored in the Covmatic digital records. This API can be run from the local Covmatic Webserver, or it can also be run as a standalone application.
CFX MANAGER SERIAL
The API communicates with the BioRad PCR machine (via the BioRad application, CFX Manager/Maestro) by exchanging XML files (via USB serial connection) that follow a defined schema. The code for this BioRad API is written in C# following the. The Covmatic digital webserver then reconciles the contents of each well in the 96-well PCR plate to the patient from whom the sample was extracted. Signals the Covmatic digital server that the json file is ready to be picked up.Interprets the results of PCR run and consolidates it into a single JSON file.Allows the user to control the BioRad CFX96 PCR with C1000 Touch or C1000 thermal cycler.A GOOD AND RECOMMENDED STARTING POINT IS TO REFER TO THE DOCUMENTATION in this repository.
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